華聯生物科技專注於基因檢測平臺的開發,具備基因微陣列晶片、次世代定序、大數據分析等基因檢測分析技術,可滿足您表達譜分析的多樣需求。我們擁有專業認證實驗室、專業技術人員、高規基因檢測設備,為您提供最完整的科學研究與基因檢測服務。

OneArray? 基因表達分析服務

Phalanx Biotech Group的生物晶片服務實驗室使用我們專有的OneArray?平臺進行基因表達分析服務。基於原廠自行研發及製造的優勢,此平臺已經過不斷的驗證,測試和更新,因此我們可以以實惠的價格為您帶來最佳的基因表達解讀。此外,由於擁有多年執行服務經驗,我們的專家也會協助您進行實驗設計、檢體運送、檢體品質分析及後續實驗分析。

特色

  • OneArray? gene expression profiling is available for the following species: Human (HOA), Mouse (MOA), Rat (ROA), Rice (RiOA), and Yeast (YOA).
  • 60-mer probes were engineered using specific lengths to match melting temperatures for superior hybridization performance.
  • Microarray service provider for 10+ years.
  • Outstanding technical support from microarray experts.
  • Professional lab technicians with 70+ years of combined experience.
  • Our complete microarray service workflow includes RNA QC, amplification, labeling, hybridization, scanning, data extraction, and data analysis.

總覽

Species available:
SpeciesOneArray PlatformNo. of Genome Probes
HumanHOA v728,264
MouseMOA v226,423
RatROA v220,715
RiceRiOA v121,179
YeastYOA v16,958
Workflow:
StepMethodQC metrics
RNA isolation (optional)Trizol/RNeasy/etc.Need > 3ug
RNA QCNanoDrop260/230 > 1.5, 260/280 > 1.8
?Agilent BioanalyzerRIN > 6
RNA amplificationEberwine Method with aa-dUTP incorporationInternally validated thresholds for aRNA yield measured with NanoDrop
RNA labelingCy-dye coupling to aa-dUTPInternally validated thresholds for labeling efficiency measured with NanoDrop
HybridizationPhalanx Method?
Data extractionAgilent Extended Dynamic RangeInternally validated metrics for hybridization efficiency using control microarray probes
Data Analysis:
  • Data normalization
  • Identify differentially expressed genes (unlimited statistical comparisons)
  • Principal components analysis
  • Hierarchical clustering
  • Gene Ontology and Pathway enrichment analyses (for each list of differentially expressed genes)

資源

OneArray? miRNA表達分析服務

Phalanx Biotech Group的生物晶片服務實驗室使用我們專有的OneArray?平臺進行miRNA表達分析服務。基於原廠自行研發及製造的優勢,此平臺已經過不斷的驗證,測試和更新,因此我們可以以實惠的價格為您帶來最佳的miRNA表達解讀。再者,由於擁有多年執行服務經驗,我們的專家也會協助您進行實驗設計、檢體運送、檢體品質分析及後續實驗分析。

特色

  • OneArray? miRNA expression profiling is available for the following species: Human (HmiOA v7), Mouse & Rat (MRmiOA v7), and Model Plant species (MPmiOA v3)
  • Probes contain miRNA-specific sequences and a proprietary spacer designed to enhance the hybridization sensitivity to miRNAs.
  • Microarray service provider for 10+ years.
  • Outstanding technical support from microarray experts.
  • Professional lab technicians with 70+ years of combined experience.
  • Our complete microarray service workflow includes RNA QC, miRNA enrichment, labeling, hybridization, scanning, data extraction, and data analysis.

總覽

Species available:
SpeciesFormatAgilent Design IDNo. of Genome Probes
Human8 x 60K07236326,083 Genes 30,606 lncRNAs
Mouse8 x 60K07480927,122 Genes 4,578 lncRNAs
Rat8 x 60K07403630,584
Arabidopsis4 x 44K02116943,803
Barley4 x 44K02162343,803
Bovine4 x 44K02364743,803
Brassica4 x 44K02252043,803
C. elegans4 x 44K02018643,803
Canine4 x 44K02119343,803
Chicken4 x 44K02644143,803
Cotton4 x 44K02252343,803
Drosophila4 x 44K02179143,803
E. coli8 x 15K02009715,208
Horse4 x 44K02132243,803
Magnaporthe4 x 44K01506015,170 Magnaporthe 6,325 Rice
Mosquito4 x 44K02044943,803
Porcine4 x 44K02644043,803
Rabbit4 x 44K02090843,803
Rhesus4 x 44K02680643,803
Rice4 x 44K01524143,803
Salmon4 x 44K02093843,803
Sheep8 x 15K01992115,208
Tobacco4 x 44K02111343,803
Tomato4 x 44K02227043,803
Wheat4 x 44K02229743,803
Xenopus4 x 44K02344843,803
Yeast8 x 15K0163226,256
Zebrafish4 x 44K02643743,803
Workflow:
StepMethodQC metrics
RNA isolation (optional)Trizol/RNeasy/etc.Need > 0.5ug
RNA QCNanoDrop260/230 > 0.7, 260/280 > 1.8
?Agilent BioanalyzerRIN > 7
RNA amplification and labelingAgilent Low Input Quick Amp Labeling KitInternally validated thresholds for aRNA yield and labeling efficiency measured with NanoDrop
HybridizationAgilent ProtocolSee below
Data extractionAgilent Extended Dynamic RangeInternally validated metrics for hybridization efficiency using control microarray probes
Data Analysis:
  • Data normalization
  • Identify differentially expressed genes (unlimited statistical comparisons)
  • Principal components analysis
  • Hierarchical clustering

資源

安捷倫生物晶片服務

Phalanx Biotech Group除了本身的產品之外,也提供相關行業領先者–安捷倫生物晶片服務,內容包含28種模式生物。由於擁有多年執行服務經驗,我們的專家會協助您進行實驗設計、檢體運送、檢體品質分析及後續實驗分析。

特色

  • OneArray? gene expression profiling is available for the following species: Human (HOA), Mouse (MOA), Rat (ROA), Rice (RiOA), and Yeast (YOA).
  • 60-mer probes were engineered using specific lengths to match melting temperatures for superior hybridization performance.
  • Microarray service provider for 10+ years.
  • Outstanding technical support from microarray experts.
  • Professional lab technicians with 70+ years of combined experience.
  • Our complete microarray service workflow includes RNA QC, amplification, labeling, hybridization, scanning, data extraction, and data analysis.

總覽

Species available:
SpeciesFormatAgilent Design IDNo. of Genome Probes
Human8 x 60K07236326,083 Genes 30,606 lncRNAs
Mouse8 x 60K07480927,122 Genes 4,578 lncRNAs
Rat8 x 60K07403630,584
Arabidopsis4 x 44K02116943,803
Barley4 x 44K02162343,803
Bovine4 x 44K02364743,803
Brassica4 x 44K02252043,803
C. elegans4 x 44K02018643,803
Canine4 x 44K02119343,803
Chicken4 x 44K02644143,803
Cotton4 x 44K02252343,803
Drosophila4 x 44K02179143,803
E. coli8 x 15K02009715,208
Horse4 x 44K02132243,803
Magnaporthe4 x 44K01506015,170 Magnaporthe 6,325 Rice
Mosquito4 x 44K02044943,803
Porcine4 x 44K02644043,803
Rabbit4 x 44K02090843,803
Rhesus4 x 44K02680643,803
Rice4 x 44K01524143,803
Salmon4 x 44K02093843,803
Sheep8 x 15K01992115,208
Tobacco4 x 44K02111343,803
Tomato4 x 44K02227043,803
Wheat4 x 44K02229743,803
Xenopus4 x 44K02344843,803
Yeast8 x 15K0163226,256
Zebrafish4 x 44K02643743,803
Workflow:
StepMethodQC metrics
RNA isolation (optional)Trizol/RNeasy/etc.Need > 0.5ug
RNA QCNanoDrop260/230 > 0.7, 260/280 > 1.8
?Agilent BioanalyzerRIN > 7
RNA amplification and labelingAgilent Low Input Quick Amp Labeling KitInternally validated thresholds for aRNA yield and labeling efficiency measured with NanoDrop
HybridizationAgilent ProtocolSee below
Data extractionAgilent Extended Dynamic RangeInternally validated metrics for hybridization efficiency using control microarray probes
Data Analysis:
  • Data normalization
  • Identify differentially expressed genes (unlimited statistical comparisons)
  • Principal components analysis
  • Hierarchical clustering

資源

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