次世代定序(NGS)是觀察您所研究物種之遺傳圖譜的有力工具。透過不同的表現型(Phenotype), 您可以尋找相關SNP、變異或致病位點;進一步瞭解藥物作用如何調控整個基因組編碼區的表達;亦或是發現新的轉錄序列;甚至探索全新的生物遺傳組成。

Phalanx Biotech Group使用Illumina / BGI平臺提供NGS服務,協助您取得一系列的生物實驗數據,包含基因組學(Genomics)、轉錄組學(Transcriptomics)、表觀遺傳學(Epigenomics)和宏觀基因組學(Metagenomics)等。我們可以依照您的需求,提供您實驗設計、檢體準備、定序、分析…等的各種協助。

NGS服務

RNA-Seq

RNA-Seq Service

RNA sequencing is a type of next-generation sequencing used to target regions of the genome that are transcribed into
messenger RNA (mRNA) and may subsequently translate into protein. RNA-seq is used to identify and quantify
transcriptional regulation of the genome.

Protocols specific to the library preparation stage are used to isolate mRNA and rid samples of rRNA, which comprises
approximately 80% of the RNA pool.

Typical sequencing configurations:
ConfigurationmRNA-seq
Library Preparation ProtocolStrand-specific, polyA Selection, paired-end
Recommended Throughput20-40 million reads per sample
PlatformIllumina HiSeq

*Sequencing configuration is project specific.

Sample submission requirements for RNA-Sequencing:
SAMPLE TYPEAMOUNTRINCONCENTRATION
Total RNA in Rnase-free water, on dry ice2ug (min > 500ng)6.050 ng/uL
RNA-Seq Applications:

RNA-seq may be used for gene expression profiling, identification of novel transcripts, identification of alternative splice events and transcriptome assembly.

Gene expression profiling by way of RNA-seq is utilized in drug development experiments, cancer research, gut microbiome studies and many more. Comparative analysis of transcript abundance between experimental groups provides unique insight into the interplay between gene and environment.

Small RNA-Seq

Small RNA-Seq Service

Small RNA sequencing is used to target small RNA molecules including: miRNA, ncRNA, snRNA, etc.

Common sequencing configurations:
Configurationsmall RNA-seq
Library Preparation ProtocolCustomizable size selection using small RNA library prep kits
Recommended Throughput7-10 million reads per sample
PlatformIllumina NextSeq
Sample submission requirements for Small RNA-Sequencing:
Sample TypeMinimum AmountRINMinimum Concentration
Total RNA200 ng>7.020 g/uL

ChIP-Seq

ChIP-Seq Sample submission requirements:
Sample TypeMinimum AmountMinimum Concentration
DNA5 ng1 ng/uL
RNA QC Requirements:
  • A260/280 > 2
  • A260/230 = 2.0~2.2
Recommended Sequencing Specifications:
  • Single end 1x50 bp
  • 20M, 40M, 75M, or 100M reads

16s Metagenomics

Smple submission requirements:
Sample TypeMinimum AmountMinimum Concentration
DNA12.5 ng1.5 ng/uL
RNA QC Requirements:
  • A260/280 > 2
  • A260/230 = 2.0~2.2
Recommended Sequencing Specifications:
  • Paired end 2x300bp
  • 100K coverage

Whole Exome Sequencing (WES)

Sample submission requirements:
Sample TypeMinimum AmountMinimum Concentration
DNA12.5 ng1.5 ng/uL
RNA QC Requirements:
  • A260/280 > 2
  • 260/230 = 2.0~2.2
Recommended Sequencing Specifications:
  • aired end 2x300 bp
  • 100K coverage

特色

  • 完整的工作流程:我們可以依照您的需求,執行樣品核酸提取、建庫(library construction)、定序、基礎數據分析和進階數據分析。
  • 低產量樣品:如果您的樣品類型為有限的RNA / DNA,請聯繫我們以了解我們能提供的特殊專屬流程。
  • 種類繁多:我們處理過各樣基因組(人類,小鼠,大鼠,倉鼠,細菌等)物種的樣本。
聯絡我們
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